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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Derivation and propagation of spermatogonial stem cells from human pluripotent cells

Fig. 5

Xeno-transplantation of SSCLCs into the seminiferous tubules of recipient mice following busulfan treatment. a H&E staining or immunostaining of mouse testes at 5 weeks after SSCLCs transplantation. Immunostaining with VASA (green) and hNuclei (red) and PNA (red). Bright arrows indicate transplanted SSCLCs. The contralateral testes without grafts were used as internal control which was named as hES2-Con and hiPS-Con. Normal testis without busulfan treatment were used as positive control. Mouse IgG/corresponding Alexa Fluor 594, Rabbit IgG/corresponding Alexa Fluor 488, and no PNA staining were used as negative control. Pound keys (#) represent the seminiferous tubules with more VASA-positive cells, and asterisk (*) represents the seminiferous tubules with fewer VASA+ cells. Scale bars, 50 μm. b Quantification of seminiferous tubules with VASA+ cells or PNA+ cells in testes of different groups. STs represent seminiferous tubules. Five to six representative sections of testes were counted. At least three mice were used in every group. Data are represented as mean ± SEM

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