Fig. 2

In vitro analysis of islet survival and function in normal conditions: a Representative confocal images, and results of b live/dead, c MTT, and d GSIS assays of tested groups (i.e., Islets, e-Islets, m-Islets, em-Islets, and emf-Islets). Confocal images and live/dead assay have been performed at days 1 and 7, and MTT and GSIS assays at day 7, in culture. Blue: live cells stained with Hoechst. Red: dead cells stained with PI. Green: AD-MSCs stained with FDA. Scale bar = 50 μm. Significant differences: b–d ^aP < 0.05: Islets vs. m-Islets or em-Islets or emf-Islets; ^bP < 0.05: m-Islets vs. em-Islets or emf-Islets; ^dP < 0.05: em-Islets vs. emf-Islets (one-way ANOVA post hoc Tukey test). b *P < 0.05: day 1 vs. day 7; d *P < 0.05: high glucose vs. low glucose (two (b, d) or one (c)-way ANOVA post hoc Tukey test)