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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing

Fig. 2

The characterization of the CHS/ADM scaffold and its effect on the survival and differentiation of MSCs. a FT-IR absorption spectra of the CHS, ADM, and CHS/ADM scaffolds. b The percent degradation mass of the ADM and CHS/ADM scaffolds after enzymatic degradation at different time points (**p < 0.01, *p < 0.05, n = 3). c SEM images showing the morphologies of the ADM and CHS/ADM scaffolds. The corresponding illustrations show 8-mm-diameter ADM and CHS/ADM scaffolds. Scale bar, 20 μm. The corresponding pore size and porosity distribution of the ADM and CHS/ADM scaffolds are shown right (n = 3). d TPEF-SHG imaging and cell counting of MSCs cultured on the ADM and CHS/ADM scaffolds for 24 h (n = 3). Scale bar, 10 μm. e Quantitative analysis of the mRNA expression levels of specific adipocyte genes (LPL, PPARγ) and osteogenic genes (Runx-2, ALP) of MSCs seeded on either the ADM and CHS/ADM scaffolds or only in medium as the control on days 14 and 21

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Stem Cell Research & Therapy

ISSN: 1757-6512