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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing

Fig. 3

Cell protective capacity of the CHS/ADM scaffold under ROS microenvironment. a Live/dead staining images of the MSCs-ADM and MSCs-CHS/ADM scaffolds with H2O2 treatment (above). Control is MSCs-ADM scaffold untreated with H2O2. Green and blue represent lived cells and dead cells, respectively. Red represents SHG imaging of scaffolds. Scale bars are 200 μm (n = 3). Cell viability (%) of the MSCs-ADM and MSCs-CHS/ADM scaffolds treated with H2O2 or untreated with H2O2 (below). b Dihydroethidium (DHE) staining images of intracellular ROS in MSCs seeded on the ADM and CHS/ADM scaffolds with H2O2 treatment. Control is MSCs-ADM scaffold untreated with H2O2. Red and blue represent ROS and nuclei, respectively. The quantitative analysis of the relative DHE intensity (n = 5) is in below. Scale bars are 200 μm; ***p < 0.001, **p < 0.01, *p < 0.05

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