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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing

Fig. 5

The CHS/ADM scaffold enhanced MSC retention in vivo. b TPEF-SHG imaging of MSCs that seeded on the CHS/ADM scaffolds transplanted into the wound site on days 7, 14, 21, and 28. Green and red represent GFP+-MSCs and collagen, respectively. a ADM scaffold as the comparison group (original magnification, × 60; scale bar, 10 μm). c The change in the number of MSCs in the wounds on days 7, 14, 21, and 28 after transplantation. d SHG intensity of collagen at different time points with increased treatment in the ADM and CHS/ADM scaffold groups. e Comparison of GFP levels in each group (7, 14, 21, and 28 days) via PCR and f western blot analysis. Statistical analysis of the expression of GFP. Data are expressed as the mean values, and the error bars represent the SD (n = 5). ***p < 0.001, **p < 0.01, *p < 0.05

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