Fig. 1

Schematic representation of the stepwise differentiation of iPSC to choroidal endothelial cells. (a) Fibroblasts obtained from patient biopsies were reprogrammed to induced pluripotent stem cells (iPSCs). iPSCs were transduced with a lentiviral vector (b) expressing the endothelial cell-specific promoter CDH5 driving expression of GFP and zeocin resistance. Forty-eight hours post-transduction, cells were placed under selection with blasticidin to select for positively transduced iPSCs. Following selection, iPSC colonies were expanded prior to stepwise choroidal endothelial cell (CEC) differentiation as outlined in (c)