Fig. 2

Differentiation of induced pluripotent stem cells into choroidal endothelial cells. Representative light microscope images acquired during the stepwise differentiation of induced pluripotent stem cells into mesoderm-derived choroidal endothelial cells. (a) iPSC colonies are expanded prior to differentiation and embryoid body (EB) formation. EBs were kept in suspension for 5 days. EB appearance after 1 (b), 3 (c), and 5 days (d) post-formation. On day 5, EBs were adhered to a Matrigel-coated 6-well plate at a density of 30 EBs per well. After 24 h (i.e., differentiation day 6), EBs have stuck to the Matrigel-coated surface (e). EBs gave rise to a heterogenous population of cells over 14 days. Areas of EC with cobblestone morphology can be seen at days 7 (f), 12 (g), and 14 (h). Note that red square in (f) and (g) demarcates the area depicted in the corresponding high-magnification inlay. Scale bars = 1000 μM