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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Stepwise differentiation and functional characterization of human induced pluripotent stem cell-derived choroidal endothelial cells

Fig. 5

iPSC-derived choroidal endothelial cells form vascular tubes in Matrigel and can migrate into decellularized human choroid extracellular matrix. Microscopy images demonstrate formation of vasculature 1 (a, e), 3 (b, f), 19 (c, g), and 22 h (d, h) post-seeding of iPSC-derived CECs into a 3D Matrigel matrix (a–d): CD31 MACS sorted cells; (e–h): zeocin selected cells. Calcein was used at the 22-h time point (d, h) to demonstrate cell viability. Scale bars = 1000 μM. (i) Transmission electron micrograph of iPSC-derived CEC tube demonstrates lumen formation. (j) Immunofluorescence labeling of human RPE/choroid after decellularization. Anti-collagen IV (red) highlights the structure of the choroid tissue following decellularization, and the absence of nuclear DAPI (blue) demonstrates the absence of native endothelial cells. iPSC-derived CECs are observed within the vascular tubes of the choriocapillaris (k, l) in both a brightfield and anti-collagen IV labeled image

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