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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β1/Smad3 signaling pathway to inhibit ovarian fibrosis to repair ovarian function in POI rats

Fig. 5

Theca cell differentiation and fibrosis expression in cultured ovarian stromal cells. a, ×40: The morphology of stromal cells under a microscope. b, ×200: The isolated cells were characterized with the immunofluorescence. b a1–a3: The mesenchymal cells were stained by Vimentin as green. b, b1–c3: The endothelial cells were stained with Factor VIII as red, and the epithelial cells were stained with cytokeratin as red. The cell nucleus was stained by DAPI as blue. c, d Cell viability of stromal cells was measured after different concentrations of CDDP or TGF-β1 inhibitor SB431542 treatment. e The expression of Cyp17a1 and α-SMA in stromal cells by immunofluorescence staining. The nucleus was stained by DAPI as blue. The theca cells were stained with Cyp17a1 as green. The MFB were stained with α-SMA as red. fg Quantitative intensity of Cyp7a1 and α-SMA staining in each group. Data are expressed as the means ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. CDDP cisplatin, Cyp17a1 Cytochrome P450 17A1, α-SMA alpha smooth muscle actin, DAPI 4,6-diamino-2-phenyl indole, and MFB myofibroblast

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