Fig. 3

Overexpressed miR-375 impedes cervical cancer cell proliferation, migration, and invasion while stimulating cell apoptosis in vitro. a The transfection efficiency of miR-375 was detected by RT-qPCR in C33A and HeLa cells. b Proliferation of C33A and HeLa cells transfected with miR-375 mimic or inhibitor was determined using EdU assay. c Migration and invasion of C33A and HeLa cells transfected with miR-375 mimic or inhibitor were determined using Transwell assay. d Apoptosis of C33A and HeLa cells transfected with miR-375 mimic or inhibitor was determined using TUNEL assay. e–h The expression of cell apoptosis-related proteins (Bcl-2 and Bax), cell cycle-related proteins (CDK4 and Cyclin D1), and cell migration-related proteins (E-cadherin and Vimentin) was assessed by Western blot analysis in C33A and HeLa cells transfected with miR-375 mimic or inhibitor, normalized to β-actin. The measurement data are presented as mean ± SD. Two groups of data are compared by an independent sample t test. *p < 0.05 compared with the NC group. The cellular experiment was repeated three times independently