Fig. 7

BMSC- EV-derived miR-375 retards xenograft tumor growth in nude mice. a Tumor size of nude mice treated with PBS, or EVs isolated from BMSCs transfected with miR-375 or miR-NC (three samples presented in each group). b Tumor volume of nude mice treated with PBS, or EVs isolated from BMSCs transfected with miR-375 or miR-NC. c Tumor weight of nude mice treated with PBS, or EVs isolated from BMSCs transfected with miR-375 or miR-NC. d The expression of miR-375 was determined using RT-qPCR in tumor tissues of mice treated with PBS, or EVs isolated from BMSCs transfected with miR-375 or miR-NC, normalized to U6. e The protein expression of MELK, Cle-caspase, and Ki67 was measured by Western blot analysis in tumor tissues of mice treated with PBS, or EVs isolated from BMSCs transfected with miR-375 or miR-NC, normalized to β-actin. The measurement data are presented as mean ± SD. Multiple groups of data are compared by one-way ANOVA and Tukey’s test. Data among multiple groups at different time points are compared using repeated measures ANOVA and Bonferroni’s test. *p < 0.05 compared with the mice treated with PBS. #p < 0.05 compared with the mice treated with EVs isolated from BMSCs transfected with miR-NC