Fig. 5

miR-27b inhibits hypertrophic differentiation of hBMSC-derived chondrocytes and upregulates SOX9 expression. a Protein levels of CBFB and RUNX2 in coimmunoprecipitates. b After sh-CBFB and sh-SC transfection, on 14 days mRNA expression of CBFB, RUNX2, COL10, SOX9, and COL2 as measured by RT-qPCR. *P < 0.05, **P < 0.001. c Protein levels of CBFB, RUNX2, SOX9, and COL10 as measured by western blot. d Semi-quantification of western blot data. *P < 0.05, **P < 0.001. e IHC staining of COL2, SOX9, and COL10 expression in chondrogenic pellets transfected with NC, sh-SC, and sh-CBFB. Scale bar = 20 μm. f Measurement of optical density for COL 2, SOX9, and COL10 was evaluated by ImageJ software, and the data represented the expression levels of COL 2, SOX9, and COL10. *P < 0.05