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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Optimal H2O2 preconditioning to improve bone marrow mesenchymal stem cells’ engraftment in wound healing

Fig. 2

Optimal H2O2 preconditioning provides anti-apoptotic protection to BMSCs. a Representative images of flow cytometric analysis by annexin V-FITC/PI dual staining after BMSCs treated for 24 h with various concentrations of H2O2 (25, 50, 100, 150, 200, 300, and 500 μM). The bottom right quadrant represents annexin V-FITC-stained cells (early-phase apoptotic cells), and the top right quadrant represents PI- and annexin V-FITC-dual-stained cells (late-phase apoptotic/necrotic cells). b Apoptotic cells are represented as the percentage of annexin V single-positive plus annexin V-FITC/PI double-positive cells (n = 3). c Representative images of flow cytometric analysis by annexin V-FITC/PI dual staining. Confluent BMSCs were pretreated with or without 50 μM of H2O2 for 12 h. After removing the supernatants, cells were incubated with fresh medium in the presence of various concentrations of H2O2 (200, 250, 300, and 500 μM) for an additional 12 h. d Apoptotic cells are represented as the percentage of annexin V single-positive plus annexin V-FITC/PI double-positive cells (n = 3). *P < 0.05, **P < 0.01 vs. the control groups (0 μM H2O2) or the H2O2 un-pretreatment group

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