Fig. 6
Optimal H2O2 preconditioning enhances BMSC tissue engraftment and would closure. The full-thickness wounds were created at the dorsum of mice, followed by the tail vein injection of PBS and DiI-labeled BMSCs with or without H2O2 preconditioning for 12 h, respectively. a, b Histological analysis of the number of transplanted DiI-labeled BMSCs (red) migrating into newly formed granulation tissue at days 1 and 3 post-wounding. Scale bar, 100 μm. Data are presented as the total cells per field (× 200) ± SEM (n = 5). **P < 0.01 vs. the BMSCs group; ΔΔP < 0.01 vs. the day 1 post-wounding within the same group. c Gross view of excisional wounds at days 0, 5, 7, 10, 13, and 15 post-wounding. d The rate of wound closure after BMSCs engrafting into the wounds. Data are shown as means ± SEM (n = 5). e, f Western blot analysis of the expressions of VEGF of wound tissue after transplantation of BMSCs. Data are shown as means ± SEM (n = 3). *P < 0.05 vs. the PBS group; ΔP < 0.05 vs. the BMSCs group