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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Multipotent adult progenitor cells grown under xenobiotic-free conditions support vascularization during wound healing

Fig. 1

XF-hMAPC cells induced an elaborate host vascular network in an in vivo Matrigel implantation assay. ad Brightfield images of implants at lower (a, c implant borders are lined by dashed white lines) and higher magnification (b, d) containing PBS (a, b) or XF-hMAPC cells (“XF”; c, d). Clearly, vascularized areas and vascular leakage are indicated by white arrowheads in panel c or b, respectively. e Cross-section of an XF implant stained with anti-human (h)CD34 in green. Positive cells are indicated by white arrowheads. f Pie diagrams representing the fraction of mice with more (blue) or less (red) than 50% of the examined sections containing vessels for the PBS (left), or XF (right) group. gi Cross-sections stained for mouse (m)CD31 in green for the PBS (g; open circles in i; n = 6), or XF (h; open triangles in i; n = 4) groups and the corresponding quantification in i. Data represent the mean fractional CD31+ area expressed as % ± S.E.M. (*P < 0.05 versus PBS by unpaired Student’s t test). DAPI was used as nuclear counterstaining (in blue) in e, g, h. Magnifications at which pictures were taken: × 10 in g, h; × 40 in e. Scale bars: 1.3 mm in a, c; 200 μm in b, d; 50 μm in g, h; and 20 μm in e

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