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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Multipotent adult progenitor cells grown under xenobiotic-free conditions support vascularization during wound healing

Fig. 6

XF-hMAPC cells dose-dependently induced an elaborate and mature vascular network in the wound center late during wound healing. ad Cross-sections representing the wound center stained for mouse (m)CD31 in green for the Plasma-Lyte control (“CTRL”; a; black circles in d; n = 14), middle-dose XF-hMAPC cell (“XF2”; b; blue triangles in d; n = 13), and high-dose XF-hMAPC cell (“XF3”; c; red triangles in d; n = 15) groups and the corresponding quantification in d. Data represent the vascular ingrowth relative to the wound length expressed as % ± S.E.M. (*P < 0.05 versus indicated condition by one-way ANOVA). Red horizontal lines in ac indicate distance covered by the vascular fronts, white arrowheads in the overview panels in the bottom indicate wound borders. eh Cross-sections zooming in on the wound center stained for mCD31 in green for CTRL (e; black circles in h; n = 15), XF2 (f; blue triangles in h; n = 16), and XF3 (g; red triangles in h; n = 16) groups and the corresponding quantification in h. Data represent the mean fraction of mCD31+ vessels expressed as number per area (in mm2) ± S.E.M. (*P < 0.05 versus indicated condition by one-way ANOVA). il Cross-sections (same as in eg) zooming in on the wound center stained for α-smooth muscle-actin (αSMA) in red for CTRL (i; black circles in l; n = 15), XF2 (j; blue triangles in l; n = 16), and XF3 (k; red triangles in l; n = 16) groups and the corresponding quantification in l. Data represent the mean fraction of αSMA+ vessels expressed as % of mCD31+ vessels ± S.E.M. (*P < 0.05 versus indicated condition by one-way ANOVA). Dermo-epidermal junction is indicated by white dotted lines in eg and ik. DAPI was used as nuclear counterstaining (in blue) in ac, eg, and ik. Magnifications at which pictures were taken: × 2.5 in ac (bottom); × 10 in ac (top); × 20 in eg, ik. Scale bars: 1 mm in ac (lower panels), 500 μm in ac (upper panels), 100 μm in eg and ik

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