Fig. 3

Establishment and characterisation of GMP-compatible lt-NES. a Representative phase contrast showing morphology of lt-NES derived from clinical grade MasterShef 7 (NES7) and 8 (NES8). Scale bars 20 μm. NES7 and NES8 cells were immunostained for the lt-NES markers Nestin, SOX2, Dach1, PLZF and the polarity marker ZO-1. Scale bars 50 μm. b Gene expression levels of lt-NES markers in NES7 and NES8 compared to research-grade AF22 lt-NES line and assessed by Q-PCR (n = 3). c Recovery after thaw of NES cells at 24 h. Graph shown as mean plus SEM (n = 3). d Representative growth curve of NES7, NES8 and control AF22 cultured under GMP-compatible conditions on L521. e Normal karyology of established NES7 at passage 15 cultured in GMP-compatible system