Fig. 2
hAECs ameliorated acute renal IRI. a Morphology of hAECs was observed under bright field microscopy. Magnification, × 100 and × 400. b Flow cytometry analysis of cell surface markers SSEA4, CD324, HLA-DR, and CD146 on hAECs. The isotypes (ISO) were used as negative controls. hAECs are positive for SSEA4 and CD324 and negative for HLA-DR and CD146. c 7-day mortality rate in IRI mice with 33-min ischemia followed by vehicle (n = 19) or hAECs (n = 20) injection. *P < 0.05. d Serum creatinine concentrations in mice with 30 min ischemia followed by vehicle (n = 11) or hAECs injection (n = 13). *P < 0.05 vs IRI+Veh group, **P < 0.01 vs IRI+Veh group. e PAS staining of post-ischemic kidneys on day 1 after 30 min ischemia. Scale bar, 25 μm. Arrows indicate damaged renal tubules. f Renal pathological scores representing the degree of tubulointerstitial damage. *P < 0.05 vs IRI+Veh group. IRI+Veh: 30-min ischemia mice injected with vehicle alone (n = 3); IRI+hAECs: 30-min ischemia mice injected with 1 × 106 hAECs (n = 3). Data are shown as mean (SD)