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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human amniotic epithelial cells ameliorate kidney damage in ischemia-reperfusion mouse model of acute kidney injury

Fig. 3

Effects of intravenous injection of hAECs-EXO in mice with IRI-AKI. a Morphology of hAECs exosomes under transmission electron microscopy. Scale bar, 100 nm. b Mean diameter and concentration of hAEC exosomes analyzed by nanoparticle tracking system (NTA). Approximately 1.6 × 1010 particles were measured by NTA in hAECs exosomes, which came from a total of 48.2 × 106 hAECs. c Western blot detection of the exosome markers CD63, Flotillin, TSG101, and Alix. d 7-day mortality rate in mice with 33-min ischemia followed by vehicle (IRI+Veh, n = 11) or hAECs-EXO (IRI+hAECs-EXO, n = 12) injection. *P < 0.05. e Serum creatinine concentrations in mice with 30-min ischemia followed by vehicle (n = 5) or hAECs injection (n = 6). *P < 0.05 vs IRI+Veh group. f PAS staining of post-ischemic kidneys at day 1 after 30-min ischemia in different groups as indicated. Scale bar, 25 μm. Arrows indicate damaged renal tubules. g Renal pathological scores representing the degree of tubulointerstitial damage. *P < 0.05 vs IRI+Veh group. IRI+Veh: 30-min ischemia mice injected with vehicle alone; IRI+hAECs-EXO: 30-min ischemia mice injected with about 3 × 108 exosomes. Data are shown as mean (SD)

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