Fig. 4

Anti-apoptotic effect of hAECs or hAECs-EXO in mice with 30-min ischemia and hAECs-EXO in H/R injured HK2 cells. a Representative micrographs of TUNEL staining in different groups as indicated at day 1, day 2, day 3, and day 7 post-ischemia. Scale Bar, 25 μm. b Quantification of TUNEL-positive cells/HPF. 3 different mice were used in each group with at least 6 images were taken on each mouse kidney. *P < 0.05 vs IRI+Veh group; **P < 0.01 vs IRI+Veh group; ^#P < 0.05 vs IRI+Veh group; ^##P < 0.01 vs IRI+Veh group. c Representative Western blots showed protein expression of cleaved-caspase 3 and caspase 3 in different groups as indicated. d Graphic presentation showed the relative abundances of cleaved-caspase 3 in different groups (n = 3). *P < 0.05 vs normal control; ^#P < 0.05 vs H/R group. e Representative micrographs of Ki67 staining in different groups as indicated at day 1, day 2, day 3, and day 7 post-ischemia. Scale bar, 25 μm. f Quantification of Ki67-positive cells/HPF. 3 different mice were used in each group with at least 6 images were taken on each mouse kidney. *P < 0.05 vs IRI+Veh group; ***P < 0.001 vs IRI+Veh group; ^#P < 0.05 vs IRI+Veh group. g Representative Western blots showed protein expression of PCNA in different groups as indicated (n = 3). h Graphic presentation showed the relative abundances of PCNA in different groups. *P < 0.05 vs normal control; ^#P < 0.05 vs H/R group