Fig. 5
hAECs or hAECs-EXO prevent peritubular capillary loss in mice with 30-min ischemia. a Representative micrographs of CD31 staining in different groups as indicated at day 7 post-ischemia. Arrows indicate CD31 positive peritubular capillaries (PTCs). Scale bar, 25 μm. b Quantification of CD31-positive signal/HPF. 3 different mice were used in each group with at least 6 images were taken on each mouse kidney. *P < 0.05 vs sham group; &P < 0.05 vs IRI+Veh group; #P < 0.05 vs IRI+Veh group. c Representative Western blot analyses showed protein expression of CD31 in different groups as indicated. d Graphic presentation showed the relative abundances of CD31 in different groups. *P < 0.05 vs sham group; &P < 0.05 vs IRI+Veh group; #P < 0.05 vs IRI+Veh group (n = 3). e Growth factors expression in post-ischemic kidneys on day 1, day 2, day 3, and day 7 after hAECs or hAECs-EXO administration in mice with IRI-induced AKI. mRNA transcripts of Egf, Fgf, Hgf, Igf-1, Pdgf, and Vegf were determined by qRT-PCR. *P < 0.05 vs IRI+Veh group; #P < 0.05 vs IRI+Veh group (n = 4)