Fig. 4

ESC-treated hRPE cells had a higher proliferation rate with a faster cell cycle turnaround time. a Proportion of cell cycle distribution in C918 cells, as assessed by flow cytometry. b Expression of the cell cycle related factors in hRPE cells, as assessed by RT-qPCR. c Western blotting of cyclin proteins and p21, p27 in hRPE cells. β-actin served as the internal control. d The expression of cyclin B1, cyclin D1, and p21 in hRPE cells as determined by immunofluorescent staining. Scale bar, 50 μm. Data are means ± SDs. *P < 0.05; **P < 0.01; ***P < 0.001