Fig. 4

RNLC integration in immune compromised rd1mouse retina and further characterization. a The transplantation of RNLCs into the degenerated retina was optimized via subretinal injection as it offered higher transplantation efficiency (6.19% RNLCs in retina) than retro-orbital (4.21% RNLCs in retina as seen by flow cytometric analysis of CFSE-labelled human RNLCs cells gated in side scatter plot vs FITC. b The CFSE-labelled RNLCs could be visualized engrafted in the inner nuclear layer in rd1 retina stained with DAPI which further (c) labelled positive for human specific chromosome X using FISH probe. d qPCR was performed using GAPDH TaqMan probe to quantify the engrafted RNLCs. Around 1.2% of human cells were found to survive in the recipient mouse retina after 4 weeks of transplantation (n = 5). e IHC was performed for human-specific retinal markers in the retina of 10 days post transplanted animals. S-OPSIN-, PDE6b-, RCVRN- and NRL-positive cells were found in upper strata of INL while VSX-2-positive cells spanned across INL to GCL. Nuclei: PI (red), respective antibodies: Alexa fluor 488 (green)