Fig. 2

Relationship between the expression level of FOXQ1 and the osteogenic differentiation of mouse bone mesenchymal stem cells. A Alizarin red staining of mouse bone mesenchymal stem cells treated with osteogenic induction medium for 14 days (microscopic views: a1–d1, scale bar 200 μm; gross views: a2–d2) and the relative amount of staining (e) showed FOXQ1 expression level influence osteogenic differentiation of mouse bone mesenchymal stem cells. ALP activity assay (f) of mouse bone mesenchymal stem cells treated with osteogenic induction medium for 7 days showed a similar result. B Relative expression of Alp, Ocn, Opg, and Runx2 in mouse bone mesenchymal stem cells at different levels of FOXQ1, 7 days and 14 days after osteogenic induction. Lv5, mBMSCs with empty pGLV5 vector control; Lv3, mBMSCs with empty pGLV3 vector control; Foxq1 ↑, mBMSCs with Foxq1 overexpressing vector; Foxq1 ↓, mBMSCs with Foxq1 shRNA expressing vector. *P < 0.05, **P < 0.01, ***P < 0.001