Fig. 3

FOXQ1 promoted Wnt/β-catenin signaling. a TOP/FOP ratio of mouse bone mesenchymal stem cells expressing different levels of FOXQ1. b Western blot of nuclear and cytoplasmic beta-catenin (CTNNB1) levels in cells with FOXQ1 overexpressed and inhibited (a, C: cytoplasmic, N: nuclear, THOC1 serve as the loading control for nuclear protein, EEF1A1 serve as the loading control for both nuclear and cytoplasmic protein). Total CTNNB1 levels were also compared among groups of mouse bone mesenchymal stem cells (b); relative level of CTNNB1 normalized to loading control was evaluated for total, cytoplasmic, and nuclear CTNNB1(c). c RT-qPCR of Wnt1 (a), Wnt10a (b), and Wnt3 (c) on FOXQ1 expression. d CTNNB1 transnucleation by immunofluorescence. Scale bar for a1, b1, c1, a3, b3, c3, 200 μm; scale bar for a2, b2, c2, a4, b4, c4, 100 μm. Lv5, mBMSCs with empty pGLV5 vector control; Lv3, mBMSCs with empty pGLV3 vector control; Foxq1 ↑, mBMSCs with Foxq1 overexpressing vector; Foxq1 ↓, mBMSCs with Foxq1 shRNA expressing vector