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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Inhibition of hypertrophy and improving chondrocyte differentiation by MMP-13 inhibitor small molecule encapsulated in alginate-chondroitin sulfate-platelet lysate hydrogel

Fig. 4

Selecting the effective concentration of BICA. a MTT assay showed no adverse effect at any concentration of BICA on the metabolic activity of the hBM-MSCs cultured in pellets for 3 and 7 days. b After 28 days of chondrogenic pellet culture, the expression of MMP-13 gene at 39 μM was significantly lower than the other groups (***P ≤ 0.001). c The expression of Coll X gene at 39 μM was significantly lower than all groups, except for 1 μM and 50 μM (***P ≤ 0.001). d GAG production was significantly higher at 20 and 39 μM compared to the other groups (*, # indicate a significant difference against 39 μM and 20 μM respectively). e Total collagen content was significantly higher at 39 μM (and control) compared to the other groups (*, # indicate a significant difference against 39 μM and control respectively). f Toluidine blue and Safranin-O and fast green staining of different concentrations of BICA on MSC pellet culture

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