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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Microparticles derived from human erythropoietin mRNA-transfected mesenchymal stem cells inhibit epithelial-to-mesenchymal transition and ameliorate renal interstitial fibrosis

Fig. 1

hEPO-KMSC generation and hEPO-KMSC and hEPO-MP characterization. a Fluorescence microscopy revealed that hEPO-KMSCs expressed copGFP and b flow cytometry demonstrated efficient transfection. c RT-PCR detection of hEPO mRNA in hEPO-KMSCs and hEPO-MPs. d hEPO secretion by hEPO-KMSCs measured by ELISA on unconditioned culture media or 24 h conditioned media from MOCK- or hEPO-KMSCs. e Flow cytometry analysis of cell surface marker (CD29, CD44, CD73) and Annexin V expression on hEPO-MPs (upper panel) and MOCK-MPs (lower panel). Red lines indicate CD29, CD44, CD73, and Annexin V expression. Black lines indicate isotypic controls. Data are representative of three independent experiments. Magnification, × 400. Scale bar, 100 μm (white)

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