Fig. 4

Establishment of functional human epidermal 3D organoid culture. a Representative images show the generation of human epidermal 3D organoids (scale bar 50 μm). b Immunostaining of ITGA6 and K14 at day 2 and ITGA6, DSG1, P63, and CLDN1 at day 5 (scale bar 100 μm). c Immunostaining of ITGA6, Ki67, and K10 at day 10 (scale bar 100 μm). All biological experiments were repeated three times (n = 3, biological replicates)