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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Maternal sevoflurane exposure affects differentiation of hippocampal neural stem cells by regulating miR-410-3p and ATN1

Fig. 2

Repeated maternal exposure to 3% sevoflurane led to early NSC differentiation in fetal brains. a Western blotting images of β-tubulin III, GFAP, and nestin. b Immunofluorescence images of β-tubulin III (green) and nestin (red). Scale bar = 100 μm. c Immunofluorescence images of GFAP (green) and nestin (red). Scale bar = 100 μm. d Quantitative analysis of β-tubulin III. e Quantitative analysis of GFAP. f Quantitative analysis of nestin. g Quantification of β-tubulin III-positive cells. h Quantification of GFAP-positive cells. Values are means ± SD (n = 5/group). *p < 0.05, **p < 0.01, ***p < 0.001 compared with control (CON) group; # p < 0.05, ## p < 0.01, ### p < 0.001 compared with sevoflurane (SEV) × 1 group. One-way analysis of variance (ANOVA) followed by Tukey’s post hoc multiple comparison test was used for data analysis

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