Fig. 4

Effects of sevoflurane exposure on the expression of β-tubulin III and GFAP in fetal hippocampi on postnatal day 28. a Western blotting images of β-tubulin III and GFAP. b Quantitative analysis of β-tubulin III. c Quantitative analysis of GFAP. d Immunofluorescence images of β-tubulin III (green) in hippocampal CA1 region. Scale bar = 100 μm, 50 μm. e Immunofluorescence images of GFAP (green) in hippocampal CA1 region. Scale bar = 100 μm, 50 μm. f Quantification of β-tubulin III-positive cells. g Quantification of GFAP-positive cells. Values are means ± SD (n = 5). **p < 0.01, ***p < 0.001 compared with CON group; ##p < 0.01 compared with SEV × 1 group. One-way ANOVA followed by Tukey’s post hoc multiple comparison test was used for data analysis