Fig. 8

MiR-410-3p suppression alleviated sevoflurane-induced early NSC differentiation. a Western blotting images of β-tubulin III, GFAP, nestin, and ATN1. b RT-qPCR analysis of miR-410-3p expression. c RT-qPCR analysis of ATN1 expression. d Immunofluorescence images of β-tubulin III (green). Scale bar = 100 μm. e Immunofluorescence images of GFAP (green). Scale bar = 100 μm. f Quantitative analysis of ATN1. g Quantitative analysis of β-tubulin III. h Quantitative analysis of GFAP. i Quantitative analysis of nestin. j Quantitative analysis of β-tubulin III on day 28. k Quantitative analysis of GFAP on day 28. l Quantification of β-tubulin III-positive cells. m Quantification of GFAP-positive cells. Values are means ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared with CON group; #p < 0.05, ##p < 0.01, ###p < 0.001 compared with SEV × 3 group. One-way ANOVA followed by Tukey’s post hoc multiple comparison test was used for data analysis