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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Long noncoding RNA H19 regulates the therapeutic efficacy of mesenchymal stem cells in rats with severe acute pancreatitis by sponging miR-138-5p and miR-141-3p

Fig. 4

LncRNA H19 directly bonded with miR-138-5p and repression of miR-138 inhibited autophagy in SAP. a Relative mRNA expression of miR-138 in pancreatic tissues estimated by qRT-PCR. b Putative complementary sites within LncRNA H19 and rno-miR-138-5p were predicted by the Target Scan software. c Co-transfection of the 293T cells with LncRNA H19 and miR-138-5p mimics decreased the luciferase activity. d Agarose gel electrophoresis assay for the complex containing LncRNA H19 and biotin-coupled miR-138-5p or biotin-coupled miR-NC. e Fold enrichment of LncRNA H19 in capture assays. f Observation of localization of LncRNA H19 in the cytoplasm of MSCs by FISH assay, and the nuclei were stained blue, and LncRNA H19 was stained red (scale bar = 50 μm). g Relative mRNA expression of miR-138 in MSCs with different treatments analyzed by qRT-PCR. h–o Western blot analysis of pancreatic FAK, PDK1, AKT, p-AKT, mTOR, p-mTOR, P62, Beclin-1, and LC3 protein levels. Data represent means ± SD from at least three independent experiments. n = 6/group. *p < 0.05, **p < 0.01, ***p < 0.001. NS, not significant

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