Fig. 5
Effect of exosome treatment on target proteins altered by CoCl2. R28 cells were treated with CoCl2 (200 μM). After incubation for 9 h, the cells were treated with exosomes. a RT-PCR of target genes and Western blot analyses of b ubiquitin-proteasome system and c Wnt/β-catenin-related protein expression levels, performed using R28 lysates altered by CoCl2. Statistical significance was determined using an unpaired t test (*p < 0.05; **p < 0.005). All experiments were performed in triplicate