Fig. 1
From: Acquisition of cancer stem cell properties in osteosarcoma cells by defined factors
The transduction of OKS induced CSC properties in MG-63 cells in vitro. a qRT-PCR of previously reported markers related to CSCs of various cancers in the transduced MG-63 cells. The mRNA expression levels were normalized to those of β-actin. The mRNA expression level of MG-parental cells was set to 1. The error bars indicate the standard error of the mean: SEM. *P < 0.05. b The morphology of the transduced MG-63 cells was evaluated at 4 days after transduction by phase contrast microscopy. The transduction of OKS led to distinct morphological changes (arrows). Scale bars represent 20 μm. c Immunoblot analyses of EMT markers (E-cadherin and vimentin)