Fig. 2

Cell proliferation of the transduced and parental MG-63 cells in vitro. a The cell number of transduced MG-63 cells was counted every 4 days from day 10 to day 22 after transduction. b Cell proliferation was examined by WST-8 assay. The proliferation rate of MG-parental cells was set to 1. The error bars indicate the standard error of the mean: SEM. *P < 0.05. c Representative images of wound healing assays at 0 and 24 h. d The effects of transduction on the migration ability of MG-63 cells were determined by a wound healing assay. The migration distance (MD) in each group was calculated according to the following equation: MD = the width of the scratch at 0 h − the width of the scratch at 24 h. The MD value of the MG-parental population was used as a reference. The relative cell migration ability was determined by the following equation: relative cell migration ability = MD (MG-OKS) or MD (MG-GFP)/MD (MG-parental). e Doxorubicin-chemoresistance analysis. The viability of cells in the presence of doxorubicin was measured by WST-8 assay. The viability of the MG-parental cells at each concentration was set to 1. f mRNA level of ABCB1 was assessed by qPCR. The mRNA expression levels were normalized to those of β-actin, and the mRNA expression level of MG-parental cells was set to 1. The error bars indicate the standard error of the mean: SEM. *P < 0.05