Fig. 2
Results of robustness, repeatability, and intermediate precision parameters. a Robustness was tested with small but voluntary variations on CTV-PBMC and MSC quantity parameters. For CTV-PBMC robustness, two quantities were tested around 3 × 105: 2.8 × 105 and 3.2 × 105. CTV-PBMC were seeded alone in the same conditions as described. Variations did not show any statistical difference between CTV-PBMC groups (p value = 0.4723, Friedman test; n = 4). For MSC quantity, two PBMC/MSC ratios were tested to assess MSC quantity’s robustness, 1/10, and 1/100. Variations did not show any statistical difference between groups for the 1/100 ratio (p value = 0.4724, Friedman test, n = 4) but a statistical difference for the 1/10 ratio (p value = 0.0388, Friedman test, n = 4). The linear portion of the curve has a regression coefficient R squared = 0.9772. b Repeatability of the assay was performed by repeating six times the entire MLR method on the same MSC sample from a single donor by the same operator the same day. CTV-PBMC percent of inhibition were linear on ratios 1/10, 1/30, 1/10, and 1/300. AUC on these points was showed a CV = 6.1%. The linear portion of the curve has a regression coefficient R squared = 0.9972. c Intermediate precision was performed by repeating four times the entire MLR method on the same MSC sample from a single donor by four operators the same day. CTV-PBMC percent of inhibition were linear on ratios 1/10, 1/30, 1/10, and 1/300. The calculation of CV for the four analyses was 4.6%