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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage

Fig. 1

Characterization of mouse BMSCs. a The spindle-shaped morphology of bone marrow cells that appeared at day 7. More confluent BMSCs were observed at P4 under light microscopy. b The expression of surface marker molecules in BMSCs (P4) was analyzed and quantified by flow cytometry, and the cells were positive for MSC markers (CD29, CD44, CD73, and CD105) and negative for hematopoietic antigens (CD34 and CD45). Additionally, nonspecific fluorescence was determined by incubation with isotype-matched anti-mouse monoclonal antibodies. c Representative images of BMSC morphology (P6) and adipogenic and osteogenic differentiation visualized by Oil Red O and Alizarin Red staining, respectively. Cells treated with BM served as a control group. Scale bar = 100 μm. Data are presented as the mean ± SD (n = 3 independent experiments). Images are shown at the original magnification

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Stem Cell Research & Therapy

ISSN: 1757-6512