Fig. 4

Effect of melatonin on cell biological behaviors in BMSCs. BMSCs were incubated with different concentrations of melatonin for 24 h. BMSCs seeded in 96-well plates were treated with melatonin (ranging from 50 to 800 μM) for 24 h. a Chemical structure of melatonin (Mel). b Cell viability was determined by a CCK-8 assay. Scratch migration (c) and Transwell chemotaxis assays (d) were performed for 24 h with melatonin treatment. Scale bar = 100 μm. Cells treated with PBS served as a control group. Values are expressed as the mean ± SD (n = 3 independent experiments). ^*p < 0.05 compared with the control group. One-way ANOVA followed by the LSD test was used to analyze the significance of differences. Images are shown at the original magnification. Mel, melatonin; OD, optical density