Fig. 3

Evaluation of SC markers in hADSC in the two conditions. a Evaluation of secreted factors (NGF, BDNF, and GDNF) by hADSC cultured in classical and cell therapy-ready conditions. One-way ANOVA multiple comparisons tests were used for assessing statistical significance among the examined conditions: (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). b Gene expression of hADSC surface receptors, NTRK1 (coding for the high-affinity receptor for NGF), NTRK2 (coding for the high-affinity receptor for BDNF), NGFR (coding for the low-affinity receptor for NGF and BDNF), and GFRA1 (coding for the receptor for GDNF). c Representative fluorescence pictures of intracellular spotted signal detection of STRO-1, Nestin, GFAP, and MPZ in hADSC cultured in classical (left panel) and cell therapy-ready conditions (right panel). Experiments were conducted in three technical and biological (3 donors) repeats