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Table 2 Immunophenotype of hADSC. Hemopoietic (CD34) and endothelial (CD45) markers were negative in all culture conditions (< 2%). The MSC markers CD73 and CD90 were expressed (> 90%) in hPL-cultured cells without statistical differences among groups. CD105 was expressed in all conditions (> 90%). The isolation method did not influence marker expression. Experiments were conducted in three technical and biological (3 donors) repeats

From: Human platelet lysate as a potential clinical-translatable supplement to support the neurotrophic properties of human adipose-derived stem cells

Protein marker

Condition 1 (collagenase + FBS) (%)

Condition 2 (explant + FBS) (%)

Condition 3 (collagenase + HPL) (%)

Condition 4 (explant + HPL) (%)

CD34

0.86 ± 0.16

1.02 ± 0.05

1.04 ± 0.20

0.96 ± 0.05

CD45

1.78 ± 0.91

1.06 ± 0.06

1.45 ± 0.59

0.88 ± 0.08

CD73

86.75 ± 6.30

87.43 ± 8.16

96.76 ± 2.84

97.95 ± 1.00

CD90

76.19 ± 11.66

58.38 ± 14.61

97.97 ± 1.39

96.33 ± 3.04

CD105

96.42 ± 1.00

95.89 ± 1.50

97.23 ± 0.98

97.91 ± 1.44