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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Epigenetic silencing of KLF2 by long non-coding RNA SNHG1 inhibits periodontal ligament stem cell osteogenesis differentiation

Fig. 2

SNHG1 inhibited osteogenic differentiation of PDLSCs. a SNHG1 expression in PDLSCs after infected SNHG1-lentivirus. b The mRNA expressions of KLF2 and osteoblastic marker genes Osx, OCN, and ALP were analyzed at day 14 of osteogenic differentiation after indicated treatment with GAPDH as a control. c The protein levels KLF2 and osteoblastic marker genes Osx, OCN, and ALP were analyzed at day 14 of osteogenic differentiation after indicated treatment with GAPDH as a control. d ALP staining of PDLSCs at day 14 of osteogenic differentiation after indicated treatment. e ALP activity was measured at day 14 of osteogenic differentiation after indicated treatment. f Alizarin Red staining of PDLSCs at day 14 of osteogenic differentiation after indicated treatment and quantification was shown at right. *p < 0.05 versus vector; #p < 0.05 versus vector+OM

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