Fig. 3From: Epigenetic silencing of KLF2 by long non-coding RNA SNHG1 inhibits periodontal ligament stem cell osteogenesis differentiationKLF2 knockdown inhibited osteogenic differentiation of PDLSCs. a The mRNA expressions of KLF2 in PDLSCs after treated with specific shRNAs of KLF2 for 48 h. b The mRNA expressions of KLF2 and osteoblastic marker genes Osx, OCN, and ALP were analyzed at day 14 of osteogenic differentiation after indicated treatment with GAPDH as a control. c The protein levels KLF2 and osteoblastic marker genes Osx, OCN, and ALP were analyzed at day 14 of osteogenic differentiation after indicated treatment with GAPDH as a control. d ALP staining of PDLSCs at day 14 of osteogenic differentiation after indicated treatment. e ALP activity was measured at day 14 of osteogenic differentiation after indicated treatment. f Alizarin Red staining of PDLSCs at day 14 of osteogenic differentiation after indicated treatment and quantification was shown at right. *p < 0.05 versus Sh-NC; #p < 0.05 versus sh-NC+OMBack to article page