Fig. 2

B cell proliferation assay and flow cytometric analysis of CD45Ra⁺/Foxp3⁺ regulatory B cells in vitro. a Bromodeoxyuridine cell proliferation assays showed a decrease in the proliferation of LPS-stimulated B cells when these cells were cocultured with ASCs. *P < 0.05. b Dot plots gated on lymphocyte (P1 region) and CD45Ra⁺ B cells (R1 region). Flow cytometric analysis revealed a significant increase in the proportion of the CD45Ra⁺/Foxp3⁺ regulatory B cell subset when these cells were cocultured with ASCs compared to that in the B cells alone group. c The number of CD45Ra⁺/Foxp3⁺ regulatory B cells were increased among B cells cocultured with ASCs compared to that in controls. Each experiment repeated at least three times. *P < 0.05