Fig. 1

HSPB7 was downregulated during the osteogenic differentiation of hASCs. a Relative mRNA expression of HSPB7 was measured by qRT-PCR at days 0, 1, 4, 7, 10, and 14 during the osteogenic differentiation of hASCs. b–d Relative mRNA expression of RUNX2, ALP, and OCN during the osteogenic differentiation of hASCs. e After osteogenic induction, the protein expression of HSPB7 was significantly reduced, accompanied by the increased expression of RUNX2. f The quantitative results of e by Image J software. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as a control. Data are presented as the mean ± SD (*P < 0.05, **P < 0.01, compared with day 0)