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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: HSPB7 regulates osteogenic differentiation of human adipose derived stem cells via ERK signaling pathway

Fig. 3

HSPB7 knockdown promoted the osteogenic differentiation of hASCs in vitro. a ALP staining in the HSPB7 knockdown (shHSPB7-1, shHSPB7-2) and control (shNC) groups at day 7 of osteogenic differentiation. b Histogram shows the 7-day quantification of ALP activity. c ARS staining at day 14 of osteogenic differentiation. d Relative quantitative analysis of ARS staining. e–g Relative mRNA expression of RUNX2, ALP, and OCN at day 14 of osteogenic differentiation. h, i HSPB7 knockdown increased the protein expression of OCN. j Confocal microscopy confirmed the upregulation of OCN protein expression in HSPB7 knockdown hASCs at day 14 of osteogenic differentiation. Scale bar = 50 μm. (*P < 0.05, **P < 0.01, compared with shNC)

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Stem Cell Research & Therapy

ISSN: 1757-6512