Fig. 4

Effect of CPAs on the migration of post-thaw ADSCs. a–c Cells were scratched and photographed at time 0. d–f Photographs were taken again after 12 h and g–i 24 h. a’–f’ Zoomed-in images of representative field of fresh ADSCs and ADSCs preserved with 1.0 M Tre + 20% Gly and 10% DMSO + 90% FBS at time 0 h, 12 h, and 24 h. j Quantification of the closure area is presented as the ratio of the closure area to the initial wound area. Values are expressed as the mean ± SD; N = 10. Scale bar = 100 μm. **p < 0.01; ***p < 0.001