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Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Circular RNA SIPA1L1 regulates osteoblastic differentiation of stem cells from apical papilla via miR-204-5p/ALPL pathway

Fig. 7

CircSIPA1L1 affects the expression of ALPL by serving as a miRNA sponge for miR-204-5p. a Schematic diagram of miR-204-5p with the seed sequences of wild-type circSIPA1L1 3′UTR (WT) and mutant circSIPA1L1 3′UTR (Mut). Luciferase reporter assays in 293 T illustrated that miR-204-5p could bind with circSIPA1L1. b The mutant ALPL-3′-UTR reporter plasmid was constructed. Luciferase reporter assays in 293 T showed that miR-204-5p could bind with ALPL. c Relative circSIPA1L1 expression level in SCAPs transfected with miR-204-5p mimics or inhibitor. d Cytoplasmic and nuclear fractions of cellular RNA were analyzed by qRT-PCR and expressed as a percentage of the input. U6 and GAPDH mRNA were used as reference RNAs for nuclear and cytoplasmic fractions, respectively. e, f Co-localization between miR-204-5p and circSIPA1L1 was observed by FISH in SCAPs (f). 18S and U6 were the internal control (e). Scale bar = 50 μm. *P < 0.05, **P < 0.01

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