Fig. 3

Identification of clinical-grade hUCMSCs and in vivo tracing. a Cultured hUCMSCs exhibited typical fibroblastic morphology (I) and could differentiate into chondroblasts (II), lipoblasts (III), and osteoblasts (IV). b Growth curve of hUCMSCs. c Flow cytometric analysis of hUCMSCs showed their expression of CD29⁺ CD73⁺ CD90⁺ CD105⁺ CD45⁻ CD34⁻. d After intravenous transplantation for 1 h, 4 h, 24 h, and 48 h, DIR dye-labeled hUCMSCs in peripheral blood cells (the red blood cells had been dissolved) were analyzed by flow cytometry. The peripheral blood cells (the red blood cells had been dissolved) in POI mice that received only DPBS were used as the negative control. DIR dye-labeled hUCMSCs were used as the positive control. e GFP-labeled hUCMSCs in the ovaries were detected by immunofluorescence after transplantation