Fig. 1

Morphology changes of monocyte-derived populations by ASCs. a Transwell setting of ASC and monocyte co-cultures. b Bright-field microscopy pictures of Mphs or mDCs in the presence or absence of ASCs at × 20 magnification. c FSC/SSC analysis by flow cytometry of M0, M1, and M2 Mphs, and mDCs in the absence or presence of ASCs. d FSC-Height versus FSC-Area to select singlets on mDC population (left) and 7-AAD staining to exclude dead cells (right). Data are representative of at least four independent experiments. Numbers in c represent the percentage of the gated population in the total sample. ASC, adipose-derived mesenchymal stem cell; FSC, forward scatter; mDC, mature dendritic cell; Mph, macrophage; SSC, side scatter