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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human adipose mesenchymal stem cells modulate myeloid cells toward an anti-inflammatory and reparative phenotype: role of IL-6 and PGE2

Fig. 3

Phenotypic and functional analysis of ASC-educated M1 Mphs. Flow cytometry analysis of a phagocytosis levels of Zymosan A, E. coli, or S. aureus particles labeled with pHrodo™. Average ± SEM of positive percentages and Geo Mean statistics are shown below; b surface expression of several phagocytic receptors; c surface expression of co-stimulatory molecules; d surface chemokine receptors, in M1 Mphs, in the presence or absence of ASCs; and e Average ± SEM of positive percentages and Geo Mean of the different surface markers. f OLINK analysis of the secretome of M1 and ASC-M1 Mph co-cultures. Data are representative of at least three independent experiments. The table shows the fold change of NPX between M1 Mphs + ASCs and M1 alone, together with the p value for this calculation; green indicates the upregulation of targets, and red indicates the downregulation of targets; only statistically significant changes are shown (n = 4). *p < 0.05, **p < 0.01. ASC, adipose-derived mesenchymal stem cell; CCR, C-C motif chemokine receptor; CD, cluster of differentiation; CDCP, CUB domain-containing protein; CX3CR, CX3C chemokine receptor; CXCR, C-X-C chemokine receptor; E. coli, Escherichia coli; GDNF, glial cell line-derived neurotrophic factor; HLA, human leukocyte antigen; IL, interleukin; LIF, leukemia inhibitory factor; MCP, monocyte chemotactic protein; Mph, macrophage; NPX, normalized protein expression; S. aureus, Staphylococcus aureus; TGF, transforming growth factor; TNF, tumor necrosis factor; TNFSF, tumor necrosis factor ligand superfamily member

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